Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8
نویسندگان
چکیده
منابع مشابه
Poly(3-hydroxybutyrate) synthesis genes in Azotobacter sp. strain FA8.
Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for beta-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into ...
متن کاملA Phasin with Many Faces: Structural Insights on PhaP from Azotobacter sp. FA8
Phasins are a group of proteins associated to granules of polyhydroxyalkanoates (PHAs). Apart from their structural role as part of the PHA granule cover, different structural and regulatory functions have been found associated to many of them, and several biotechnological applications have been developed using phasin protein fusions. Despite their remarkable functional diversity, the structure...
متن کاملCloning of poly (3-Hydroxybutyrate) synthesis genes from Azotobacter vinelandii into Escherichia coli
The Azotobacter vinelandii is an aerobic, nitrogen-fixing bacterium with the ability to accumulate polyesters such as poly (3-hydroxybutyrate), a particular group of the polyhydroxyalkanoates (pha), when will be cultivated on several carbon sources, including sucrose. Although the capacity of Azotobacter strains to accumulate polyesters is well known, and the genes responsible for their synthes...
متن کاملControl of polyhydroxyalkanoate synthesis in Azotobacter vinelandii strain UWD
Microbiology, University of Alberta, Edmonton, Alberta T6G 2E9, Canada polyhydroxyalkanoate (PHA) in mutant Azotobacter vinelandii UWD, the kinetic properties of 3-ketothiolase, acetoacetyl-CoA reductase, and /3hydroxybutyrate dehydrogenase were examined. The regulatiorn of the condensation of acetyl-CoA mediated by 3-ketothiolase was narmal, in that it was negatively regulated by free CoA, but...
متن کاملGenes in Azotobacter vinelandii
Azotobacter vinelandii contains a heterodimeric, membrane-bound [NiFelhydrogenase capable of catalyzing the reversible oxidation of H2. The 13 and at subunits of the enzyme are encoded by the structural genes hoxK and hoxG, respectively, which appear to form part of an operon that contains at least one further potential gene (open reading frame 3 1ORF3]). In this study, determination of the nuc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2001
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.67.11.5331-5334.2001